Research

Research Fields

* Biofuel production. Biorefineries. Biomass
* Wastewater treatment
* Olive oil production

Research Group

Ingeniería Química y Ambiental (TEP-233)

Papers

2017

-Synthetic biology and metabolic engineering approaches and its impact on non-conventional yeast and biofuel production
-Madhavan A, Jose AA, Binod P, Sindhu R, Sukumaran RK, Pandey A, Castro E.
-Front. Energy Res. 5:8 (2017)

-Combined acid/alkaline-peroxide pretreatment of olive tree biomass for bioethanol production
-José Carlos Martínez-Patiño, Encarnación Ruiz, Inmaculada Romero, Cristóbal Cara, Juan Carlos López-Linares, Eulogio Castro
-Bioresource Technology 239 (2017) 326-335

-Bifidobacterial growth stimulation by oligosaccharides generated from olive tree pruning biomass
-Encarnación Ruiz, Beatriz Gullón, Patrícia Moura, Florbela Carvalheiro, Gemma Eibes, Cristóbal Cara, Eulogio Castro
-Carbohydrate Polymers 169 (2017) 149–156

-Comparative analysis of data mining and response surface methodology predictive models for enzymatic hydrolysis of preatreated olive tree biomass
-Francisco Charte, Inmaculada Romero, Antonio Rivera, Maria Dolores Pérez, Eulogio Castro
-Computers and Chemical Engineering 101 (2017) 23-30

-Water hyacinth a potential source for value addition: an overview
-Raveendran Sindhu, Parameswaran Binod, Ashok Pandey, Aravind Madhavan, Jose Anju Alphonsa, Narisetty Vivek, Edgard Gnansounou, Eulogio Castro, Vincenza Faraco
-Bioresource Technology 230 (2017) 152-162

-Design and optimization of the sulfuric acid pretreatment of extracted olive tree biomass using response surface methodology
-José C. Martínez-Patiño, Inmaculada Romero, Encarnación Ruiz, Cristóbal Cara, Juan M. Romero-García, Eulogio Castro
-Bioresources 12 (2017) 1779-1797

2016

-Techno-economic evaluation of strategies based on two steps organosolv pretreatment and enzymatic hydrolysis of sugarcane bagasse for ethanol production
-Leyanis Mesa, Nancy López, Cristóbal Cara, Eulogio Castro, Erenio González, Solange I. Mussatto
-Renewable Energy 86 (2016) 270-279

-Bioconversion of rapeseed straw: enzymatic hydrolysis of whole slurry and co-fermentation by an ethanologenic Escherichia coli
-Energy & Fuels 30 (2016) 9532-9539
-López-Linares, Juan Carlos; Romero, Inmaculada; Cara, Cristóbal; Castro, Eulogio

-An olive tree pruning biorefinery for co-producing high value-added bio-products and biofuels. Economic and energy efficiency analysist
-J.M. Romero-García, A. Sanchez, G. Rendón-Acosta, J.C. Martínez-Patiño, E. Ruiz, G. Magaña, E. Castro
-Bioenergy Research 9 (2016) 1070-1086

-An approach to cellulase recovery from enzymatic hydrolysis of pretreated sugarcane bagasse with high lignin content
-Leyanis Mesa, Erenio González, Cristóbal Cara, Eulogio Castro, Solange I. Mussatto
-Biocatalysis and Biotransformation 33 (2016) 287-297

-Composition of secoiridoid derivatives from Picual virgin olive oil using response surface methodology with regard to malaxation conditions, fruit ripening and irrigation management
-Antonia de Torres, Francisco Espínola, Manuel Moya, Eulogio Castro
-European Food Research and Technology 242 (2016) 1709-1718

-Obtaining sugars and natural antioxidants from olive leaves by steam-explosion
-Juan Miguel Romero-Garcíba, Antonio Lama-Muñoz, Guillermo Rodríguez-Gutiérrez, Manuel Moya, Encarnación Ruiz, Juan Fernández-Bolaños, Eulogio Castro
-Food Chemistry 210 (2016) 457-465

2015

-Techno-economic evaluation of strategies based on two steps organosolv pretreatment and enzymatic hydrolysis of sugarcane bagasse for ethanol production
-Leyanis Mesa, Nancy López, Cristóbal Cara, Eulogio Castro, Erenio González, Solange I. Mussatto
-Renewable Energy 86 (2016) 270-279

-Optimization of uncatalyzed steam explosion pretreatment of rapeseed straw for biofuel production
-Juan C. López-Linares, Ignacio Ballesteros, Josefina Tourán, Cristóbal Cara, Eulogio Castro, Mercedes Ballesteros, Inmaculada Romero
-Bioresource Technology 190 (2015) 97-105

-Comparative study of coadjuvants for extraction of olive oil
-Francisco Espínola, Manuel Moya, Antonia de Torres, Eulogio Castro
-European Food Research and Technology 241 (2015) 759-768

-Hemicellulose-derived sugars solubilisation of rape straw. Cofermentation of pentoses and hexoses by Escherichia coli
-Juan C. López-Linares, Cristóbal Cara, Encarnación Ruiz, Manuel Moya, Eulogio Castro, Inmaculada Romero
-Spanish Journal of Agricultural Research 13 (3) (2015) e0213

-Ethanol production from rape straw by a two-stage pretreatment under mild conditions
-Inmaculada Romero, Juan C. López-Linares, Yaimé Delgado, Cristóbal Cara, Eulogio Castro
-Bioprocess and Biosystems Engineering 38 (2015) 1469-1478

-High solids loading pretreatment of olive tree pruning with dilute phosphoric acid for bioethanol production by Escherichia coli
-Martínez-Patino, José Carlos; Romero-García, Juan; Ruiz, Encarnación; Oliva, José Miguel; &Acutelvarez, Cristina; Romero, Inmaculada; Negro, María; Castro, Eulogio
-Energy & Fuels 29 (2015) 1735-1742

2014

-Techno-economic and environmental assessment of an olive stone based biorefinery
-Valentina Hernández, Juan M. Romero-Garcíba, Javier A. Dávila, Eulogio Castro, Carlos A. Cardona
-Resources, Conservation and Recycling 92 (2014) 145-150

-Low energy-demanding recovery of antioxidants and sugars from olive stones as preliminary steps in the biorefinery context
-Antonio Lama-Muñoz, Juan Miguel Romero-Garcíba, Cristóbal Cara, Manuel Moya, Eulogio Castro
-Ind Crops Prod 60 (2014) 30-38

-Optimization of dilute-phosphoric-acid steam pretreatment of Eucalyptus benthamii for biofuel production
-Eulogio Castro, Ismael U. Nieves, Mike T. Mullinnix, William J. Sagues, Ralph W. Hoffman, Marco T. Fernández-Sandoval, Zhuoli Tian, Donald L. Rockwood, Bijay Tamang, Lonnie O. Ingram
-Applied Energy 125 (2014) 76-83

-Recycling of washed olive pomace ash for fired clay brick production
-José A. de la Casa, Eulogio Castro
-Construction and Building Materials 61 (2014) 320-326

-Economic and environmental assessment of syrup production. Colombian case
-Javier A. Dávila, Valentina Hernández, Eulogio Castro, Carlos A. Cardona
-Bioresource Technology 161 (2014) 84-90

-Dilute sulphuric acid pretreatment and enzymatic hydrolysis of Jatropha curcas fruit shells for ethanol production
-Garcíba A, Cara C, Moya M, Rapado J, Puls J, Castro E, Martín C
-Industrial Crops and Products 53 (2014) 148-153

-Biorefinery based on olive biomass. State of the art and future trends
-J.M. Romero-Garcíba, L. Niño, C. Martínez-Patiño, C. Álvarez, E. Castro, M.J. Negro
-Bioresource Technology 159 (2014) 421-432

-Bioethanol production from rapeseed straw at high solids loading with different process configurations
-López-Linares JC, Romero I, Cara C, Ruiz E, Moya M, Castro E
-Fuel 122 (2014) 112-118

-Developing biorefineries: creating products from renewable sources in a single facility
-Eulogio Castro
-Biofuels, Bioproducts and Biorefining 8 (2014) 609–610

-Experimental study on ethanol production from hydrothermal pretreated rapeseed straw by simultaneous saccharification and fermentation
-Negro MJ, Álvarez C, Ballesteros I, Romero I, Ballesteros M, Castro E, Manzanares P, Moya M, Oliva JM
-J Chem Technol Biotechnol 89 (2014) 104–110

-Ethanol production from glucose and xylose obtained from steam exploded water-extracted olive tree pruning using phosphoric acid as catalyst
-López-Linares JC, Romero I, Cara C, Ruiz E, Castro E, Moya M
-Bioresource Technology 153 (2014) 101–107

-Restructuring the processes for furfural and xylose production from sugarcane bagasse in a biorefinery concept for ethanol production
-Leyanis Mesa, Marlen Morales, Erenio González, Cristóbal Cara, Inmaculada Romero, Eulogio Castro, Solange I. Mussatto
-Chemical Engineering and Processing: Process Intensification 85 (2014) 196-202

2013

-Pretreatment of olive tree biomass with FeCl3 prior enzymatic hydrolysis
-López-Linares JC, Inmaculada Romero, Manuel Moya, Cristóbal Cara, Encarnación Ruiz, Eulogio Castro
-Bioresource Technology 128 (2013) 180-187

-Dilute sulfuric acid pretreatment of sunflower stalks for sugar production
-Encarnación Ruiz, Inmaculada Romero, Manuel Moya, Cristóbal Cara, Juan D. Vidal, Eulogio Castro
-Bioresource Technology 140 (2013), 292-298

-Experimental study on ethanol production from hydrothermal pretreated rapeseed straw by simultaneous saccharification and fermentation
-Juan C. López-Linares, Inmaculada Romero, Cristóbal Cara, Encarnación Ruiz, Eulogio Castro, Manuel Moya
-J Chem Technol Biotechnol DOI: 10.1002/jctb.4110

-Fermentable sugar production from rapeseed straw by dilute phosphoric acid pretreatment
-López-Linares JC, Cara C, Moya M, Ruiz E, Castro E, Romero I
-Industrial Crops and Products 50 (2013), 525-531

2012

-Fired clay masonry units production incorporating two-phase olive mill waste (alperujo)
-José A. de la Casa, Inmaculada Romero, Juan Jiménez, Eulogio Castro
-Ceramics International 38, 5027-5037, 2012

-Production, purification and characterisation of oligosaccharides from olive tree pruning autohydrolysis.
-Cristóbal Cara, Encarnación Ruiz, Florbela Carvalheiro, Patrícia Moura, Ignacio Ballesteros, Eulogio Castro, Francisco Gírio
-Industrial Crops and Products 40, 225-231, 2012.

-Olive tree pruning as an agricultural residue for ethanol production. Fermentation of hydrolysates from dilute acid pretreatment.
-Manuel J. Díaz, Cristóbal Cara, Encarnación Ruiz, Inmaculada Romero, Eulogio Castro
-Spanish Journal of Agricultural Research 10 (3), 643-648, 2012.

2011

-Comparison of process configurations for ethanol production from two-step pretreated sugarcane bagasse.
-Mesa L, González E, Romero I, Ruiz E, Cara C, Castro E
-Chemical Engineering Journal 175, 185-191, 2011.

-Modeling of virgin olive oil extraction using response surface methodology.
-Francisco Espínola, Manuel Moya, Diego G. Fernández, Eulogio Castro
-Int Journal of Food Science and Technology 46, 2576-2583, 2011.

-Hydrothermal pre-treatment and enzymatic hydrolysis of sunflower stalks.
-Manuel J. Díaz, Cristóbal Cara, Encarnación Ruiz, Mercedes Pérez-Bonilla, Eulogio Castro
-Fuel 90, 3225-3229, 2011.

-Estrategias de producción de bioetanol a partir de tallos de girasol.
-Encarnación Ruiz, Juan Antonio Miralles, Cristóbal Cara, Inmaculada Romero, Manuel Moya, Eulogio Castro
-Afinidad 68 (556) nov-dic 2011, 417-423.

-Evaluación de residuales de la hidrólisis ácida del bagazo como productos de alto valor agregado.
-Marlén Morales-Zamora, Imilsis Espino-Leal, Leyanis Mesa-Garriga, Delvis Acosta-Martínez, Erenio González, Eulogio Castro
-Afinidad 68 (556) nov-dic 2011, 453-458.

-Injection of air into the headspace improves fermentation of phosphoric acid pretreated sugarcane bagasse by Escherichia coli MM170.
-Nieves IU, Geddes CC, Mullinnix MT, Hoffman RW, Tong Z, Castro E, Shanmugam KT, Ingram LO
-Bioresource Technology 102, 6959-6965, 2011

-Effect of water extraction on sugars recovery from steam exploded olive tree pruning.
-Ballesteros I, Ballesteros M, Cara C, Sáez F, Castro E, Manzanares P, Negro MJ, Oliva JM
-Bioresource Technology 102, 6611-6616, 2011

-The effect of organosolv pretreatment variables on enzymatic hydrolysis of sugarcane bagasse.
-Mesa L, González E, Cara C, González M, Castro E, Mussatto SI
-Chemical Engineering Journal 168, 1157-1162, 2011

-Organosolv pretreatment of olive tree biomass for fermentable sugars.
-Díaz M, Huijgen WJJ, van der Laan RR, Reith JH, Cara C, Castro E
-Holzforschung 65, 177-183, 2011

-Different process configurations for bioethanol production from pretreated olive pruning biomass.
-Manzanares P, Negro MJ, Oliva JM, Sáez F, Ballesteros I, Ballesteros M, Cara C, Castro E, Ruiz E.
-J Chem Technol Biotechnol 86, 881-887, 2011

-Dilute acid pretreatment of rapeseed straw for fermentable sugar generation.
-Castro E, Díaz MJ, Cara C, Ruiz E, Romero I, Moya M
-Bioresource Technology 102, 1270-1276, 2011

2010

-Hydrothermal pre-treatment of rapeseed straw.
-Díaz MJ, Cara C, Ruiz E, Romero I, Moya M, Castro E
-Bioresource Technology 101, 2428-2435, 2010

-Acid hydrolysis of olive tree biomass.
-Romero I, Ruiz E, Castro E, Moya M
-Chemical Engineering Research and Design 88, 633-640, 2010

-Industrial trials on coadjuvants for olive oil extraction.
-Moya M, Espínola F, Fernández D, Torres A, Marcos J, Vilar J, Josue J, Sánchez T, Castro E
-Journal of Food Engineering 97, 57-63, 2010

-An approach to optimization of enzymatic hydrolysis from sugarcane bagasse based on organosolv pretreatment.
-Mesa L, González E, Cara C, Ruiz E, Castro E, Mussatto S
-J Chem Technol Biotechnol 85, 1092-1098, 2010.

-Preliminary evaluation of organosolv pretreatment of sugar cane bagasse for glucose production. Application of 23 experimental design.
-Mesa L, González E, Ruiz E, Romero I, Cara C, Felissia F, Castro E
-Applied Energy 87, 109-114, 2010

-Mechanisms of lead uptake by fungal biomass isolated from heavy metals habitats.
-Ezzouhri L, Ruiz E, Castro E, Moya E, Espínola F, Cherrat L, Er-Raiouic H, Lairini K
-Afinidad 67 (545), 39-44, 2010

2009

-Valorisation of wastewater from two-phase olive oil extraction in fired clay brick production.
-de la Casa JA, Lorite M, Jiménez J, Castro E
-Journal of Hazardous Materials 169, 271-278, 2009

-Inhibition of Pichia stipitis fermentation of hydrolysates from olive tree cuttings.
-Díaz M, Ruiz E, Romero I, Cara C, Moya M, Castro E
-World Journal of Microbiology and Biotechnology 25, 891-899, 2009

-Antioxidant activity of the phenolic compounds released by hydrothermal treatments of olive tree pruning.
-Conde E, Cara C, Moure A, Ruiz E, Castro E, Domínguez H.
-Food Chemistry 114, 806-812, 2009

-Improved extraction of virgin olive oil using calcium carbonate as coadjuvant extractant.
-Espínola F, Moya M, Fernández D, Castro E.
-Journal of Food Engineering 92, 112-118, 2009

-Heavy metal tolerance of filamentous fungi isolated from polluted sites in Tangier, Morocco.
-Ezzouhri L, Castro E, Moya M, Espínola F, Lairini K
-African Journal of Microbiology Research 3, 35-48, 2009

2008

-Evaluation of steam explosion pre-treatment for enzymatic hydrolysis of sunflower stalks.
-Ruiz E, Cara C, Manzanares P, Ballesteros M, Castro E
-Enzyme and Microbial Technology 42 (2),160-166, 2008

-Conversion of olive tree biomass into fermentable sugars by dilute acid pretreatment and enzymatic saccharification.
-Cara C, Ruiz E, Oliva JM, Sáez F, Castro E.
-Bioresource Technology 99, 1869-1876, 2008

-Uptake of lead by Saccharomyces cerevisiae isolated from Tangier brewery wastewater.
-Ezzouhri L, Castro E, Moya M, Espínola F, Guerrouj M, Rossi B, Lairini K.
-European Journal of Scientific Research 19, 668-682, 2008

-Production of fuel ethanol from steam-explosion pretreated olive tree pruning.
-Cara C, Ruiz E, Ballesteros M, Manzanares P, Negro MJ, Castro E.
-Fuel 87, 692-700, 2008

-Antioxidant activity of liquors from steam explosion of Olea europea wood.
-Castro E, Conde E, Moure A, Falqué E, Cara C, Ruiz E, Domínguez H.
-Wood Science and Technology 42, 579-592, 2008

2007

-Sugar fermentation by Fusarium oxysporum to produce ethanol.
-Ruiz E, Romero I, Moya M, Sánchez S, Bravo V, Castro E.
-World Journal of Microbiology and Biotechnology 23, 259-267, 2007

-Ethanolic fermentation of phosphoric acid hydrolysates from olive tree pruning.
-Romero I, Moya M, Sánchez S, Ruiz E, Castro E, Bravo V.
-Industrial Crops and Products 25, 60-168, 2007

-Fermentation of olive tree pruning acid-hydrolysates by Pachysolen tannophilus.
-Romero I, Sánchez S, Moya M, Castro E, Ruiz E, Bravo V.
-Biochemical Engineering Journal 36, 108-115, 2007

-Liquid hot water pretreatment of olive tree pruning residues.
-Cara C, Romero I, Oliva JM, Sáez F, Castro E.
-Applied Biochemistry and Biotechnology 136-140, 379-394, 2007

2006

-Enhanced enzymatic hydrolysis of olive tree wood by steam explosion and alkaline-peroxide delignification.
-Cara C, Ruiz E, Ballesteros I, Negro MJ, Castro E.
-Process Biochemistry 41, 423-429, 2006

-Ethanol production from pretreated olive tree wood and sunflower stalks by an SSF process.
-Ruiz E, Cara C, Ballesteros M, Manzanares P, Ballesteros I, Castro E.
-Applied Biochemistry and Biotechnology 129-132, 631-643, 2006

Congress

34th Symposium on Biotechnology for Fuels and Chemicals
New Orleans, LA
April 30-May 2, 2012

Enhanced enzymatic hydrolysis of olive tree biomass by FeCl3 pretreatment
Techno-economic analysis of different process configurations for bioethanol production from pretreated olive pruning biomas
Comparison, modeling and simulation of enzymatic saccharification on olive tree biomass under dilute acid and autohydrolysis pretreatment

20th European Biomass Conference and Exhibition

Acid hydrolysis as a strategy to increase the extraction of carbohydrates from macroalgae
Comparison of olive tree biomass enzymatic hydrolysis performed on separated pretreated solids and whole slurry
Dilute sulfuric acid pretreatment and enzymatic hydrolysis of Jatropha curcas L. fruit shells for ethanol production
Cellulase Recycling for Enzymatic Hydrolysis of Pretreated Sugarcane Bagasse
Evaluation of the potential of olive tree pruning and rapeseed straw hydrolysates as fermentation media for biotechnological processes
Tools For Decision-Making Support in Biorefineries Development

33th Symposium on Biotechnology for Fuels and Chemicals
Seattle, WA
May 2-5, 2011

Fermentation of liquors from pretreated olive tree biomass by ethanologenic Escherichia coli
Sugar production from dilute acid pretreated sunflower stalks

19th European Biomass Conference and Exhibition

Comparison of process configurations for ethanol production from sunflower stalks
Enzymatic hydrolysis of sugarcane straw for ethanol production
Enzymes for ethanol production from sugarcane bagasse
Furfural, by-product of sugarcane bagasse ethanol. A simulation study
Production of value-added chemical products from solid wastes of the enzymatic hydrolysis step pf sugarcane bagasse ethanol
Techno-economic simulation of a lignocellulosic ethanol plant
Restructuring of sugarcane facilities for the combined production of bioethanol and co-products. A cuban case

32th Symposium on Biotechnology for Fuels and Chemicals
Clearwater Beach, FL
April 19-22, 2010

Liquid hot water pretreatment and enzymatic hydrolysis of sunflower stalks1
Dilute acid pretreatment and enzymatic hydrolysis of rapeseed straw​

18th European Biomass Conference and Exhibition

Presaccharification of two-step pretreated sugarcane bagasse.
Pretreatment of sugarcane straw for ethanol production.
Techno-economic comparison of different alternatives for the production of ethanol and by-products from sugarcane bagasse.
Two-step fractionation of sugarcane bagasse for ethanol production.

31th Symposium on Biotechnology for Fuels and Chemicals
S. Francisco, CA
May 3-6, 2009

LHW pretreatment and enzymatic hydrolysis of rapeseed straw

30th Symposium on Biotechnology for Fuels and Chemicals
New Orleans, LA
May 4-7, 2008

Olive tree biomass as a raw material for ethanol production. Comparison of pretreatments
Simultaneous saccharification and fermentation of pretreated olive tree pruning for bioethanol production